Operator error is the most common reason for a failed spore test. If your autoclave has automatic controls and completing cycles without giving any failure messages, the chances are that the causes of a failed spore test due to some type of operator error.

You may need to review the sterilization procedures your staff has been using to see if you could identify the problem.
Firstly you have to make sure that they are not overloading the Autoclave. Check the manufacturer’s specifications in regards to how the sterilizer should be loaded.

The next thing you will need to check is the time and temperature settings. It is possible that the factory default settings somehow got changed or the operator is simply not running the sterilizer hot enough to long enough. Manufactures default settings can vary a little but generally an autoclave has to maintain 270F degrees Fahrenheit for 12 minutes.

Lastly if the Autoclave is a manual unit with manual controls, you will need to monitor and record the temperature and pressure during the cycle. If the temperature or pressure fluctuates at all after reaching the exposure temperature you will need to contact Medicanix for service. Medicanix likes to be sure that you have had more than one failed spore test before preforming an autoclave repair service visit.

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